대학원 공지

  국제 심포지움 시리즈 
건국대학교 생명과학특성학과/대학원 생명과학과
4단계 BK21 미래통합형 생명과학 인재양성 사업단
The mechanism of the regulation of anillin mRNA subcellular localization in early Drosophila embryos
mRNA localization is one of the mechanisms of spatial and temporal regulation of protein localization. A number of studies
show that mRNA localization plays a critical role in biological phenomenon. However, the molecular mechanism and biological
function of mRNA localization has not been clarified.
 
In early Drosophila embryos, 13 times nuclear divisions are occurred without cytokinesis. Most of the nuclei move to the
surface of the embryo at nuclear division cycle 10 and those nuclei divide three more times at the surface of the embryo
simultaneously. During these four nuclear divisions, called syncytial blastoderm stage, transient invagination of plasma membrane,
known as pseudo-cleavage furrow (PCF), arise between nuclei.
We previously suggested that the localization patterns of five mRNAs (anillin, canoe, diaphanous, polychaetoid, smallish) are
similar to that of the PCF. In this study, we tried to find out the molecular mechanism of the PCF localization of anillin mRNA.
 
By in situ hybridization combined with immunocytochemistry, we revealed that anillin mRNA is localized to the tip of the
PCF throughout cell cycle during syncytial blastoderm stage. To investigate the important region in anillin mRNA for its PCF
localization, we generated several transgenic fly lines expressing DsRed-monomer fused with a set of deletion derivatives of
anillin mRNA and analyzed the localization of the exogenous mRNAs by in situ hybridization with anti-DsRed monomer probe,
respectively. The results indicate that coding sequence (CDS) is necessary and sufficient for localization to the tip of the PCF. We
carried out further localization analyses to narrow down the region required for the PCF localization in CDS. However, any anillin
mRNAs that partially deleted CDSs did not localize to the PCF. Therefore, we investigated another possibility whether the nascent
polypeptide of Anillin protein is important for its mRNA localization. In the result, it was revealed that the inhibition of
translational initiation by insertion of a stable stem-loop structure between 5’ untranslated region and CDS perturbed the PCF
localization of anillin mRNA, indicating that the PCF localization depends on its translation.
연사: Hideki Yoshida, PhD, Professor, Department of Applied Biology, Kyoto Institute of Technology, Kyoto, Japan
일시: 2021년 6월 08일 (화) 오후 5시~6시